I-Cellulase plant fibre degrading enzyme I-enzyme yebhayoloji yebanga lokudla Isithombe esifakiwe

I-Cellulase plant fiber degrading enzyme I-enzyme yebhayoloji yebanga lokudla

Incazelo emfushane:

I-Cellulase iyiqembu lama-enzyme akhishwa yi-microfiltration, i-ultrafiltration kanye ne-vacuum-freeze-dry ngemva kokuvutshelwa okujulile kwe-Trichoderma SPP ekhethiwe.Isetshenziselwa kakhulu ukwehlisa i-cellulose ukukhiqiza ushukela kanye nokucekela phansi udonga lwamaseli ezitshalo ekucubunguleni ijusi yezithelo nemifino ukuze kuthuthukiswe isivuno sejusi.Incibilika emanzini, isixazululo samanzi siwuketshezi olunsundu ngokukhanyayo.Sicela usithinte, usivumele sikunikeze isevisi engcono!

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Indlela yokwenza

I-Cellulase ngokuyinhloko iqukethe i-exonuclease β-glucanase, i-endoonuclease β-glucanase ne-β-glucosidase, kanye ne-xylanase enomsebenzi ophezulu.Indlela yokusebenza ukuthi isebenza ku-β-1, amabhondi angu-4 kusukela ngaphakathi kwe-molecule ukuze inciphise i-cellulose, futhi ikhiqize i-cellobiose kusukela ku-terminal enganciphisi i-β-1, amabhondi angu-4 we-fibrdextrin, bese i-hydrolyzes ibe yi-glucose.

Ukuncibilika

Incibilika emanzini, isixazululo samanzi siwuketshezi oluphuzi olukhanyayo.
Isingeniso Somkhiqizo:
Izithako eziyinhloko: i-cellulase, i-glucose
Imininingwane yomkhiqizo: 10-20,000 U/g
Incazelo: Impushana ensundu ngokukhanyayo
Imodi yesitoreji: yomile ekamelweni lokushisa futhi ugweme ukukhanya, izinga lokushisa eliphezulu lesitoreji (0~4℃)
Impilo yeshelufu: ivalwe ku-4 ℃ ingagcinwa izinyanga ezingama-24, i-15 ℃ ingagcinwa izinyanga eziyi-18, izinyanga eziyi-12 ekamelweni lokushisa

Inkambu yohlelo lokusebenza

1. Ukukhishwa kwezitshalo
I-Cellulase ingasetshenziswa ekukhishweni nasekucutshungulweni kwezitshalo ngokudiliza udonga lwamaseli wezitshalo nokuhlakaza izinto ezingaphakathi kweseli.Uma isetshenziselwa ukucubungula ijusi yezithelo nemifino, ukukhiqizwa kwejusi nokucaca kungathuthukiswa.Uma kuqhathaniswa nendlela yendabuko, isizinda se-enzymatic sinezinzuzo zokushisa okuphansi, ukusebenza kahle okuphezulu futhi akukho ukungcola.
2. Ukucubungula ujusi
Njengamanje, imboni yokucubungula ijusi ibhekene nezinkinga zokuvunwa kwejusi ye-pulp ephansi, ukudla isikhathi eside, ijusi elinamafu, i-viscosity ephezulu, kulula ukuyikhipha njalonjalo.I-Cellulase ingabolisa udonga lweseli yesitshalo, ithuthukise isivinini sokuphuma kwejusi kanye nezinga lokuphuma kwejusi, futhi yenze ijusi eguqubele icace kakhudlwana.
3. Ukwenza i-soy sauce
Ukwengeza i-cellulase enqubweni yokwenza i-soy sauce kungenza udonga lweseli lezinto zokusetshenziswa zesoya luthambe, lunwebe futhi lubhubhise, futhi lukhulule amaprotheni nama-carbohydrate avaleleke emangqamuzaneni, angakwazi nje ukuthuthukisa ukugcwala kwesoso yesoya, athuthukise ikhwalithi ye-soy sauce. soy sauce, kodwa futhi unciphise umjikelezo wokukhiqiza futhi uthuthukise ukukhiqiza.
4. Imboni yokuphisa utshwala
Ukwengezwa kwe-vitaminase ekuvutshelweni kwewayini kungakhuphula kakhulu isivuno sotshwala kanye nokusetshenziswa kwempahla eluhlaza, kunciphise ukubukeka kwesixazululo, futhi kunciphise isikhathi sokuvutshelwa.I-Cellulase ingabolisa udonga lwamaseli ezitshalo futhi ikhiqize i-glucose ukuze isetshenziswe imvubelo.Ngesikhathi esifanayo, kuyasiza ekukhululweni nasekusetshenzisweni kwesitashi futhi kuthuthukise isivuno sewayini.
5. Ukucubungula okuphakelayo
Imfuyo evamile kanye nokudla kwezinkukhu, okufana nokusanhlamvu, ubhontshisi, ukolweni kanye nemikhiqizo ekhiqizwayo, iqukethe inani elikhulu le-cellulose.Ngaphezu kwezilwane ezelusayo zingasebenzisa amanye amagciwane e-rumen, ezinye izilwane ezinjengezingulube, izinkukhu nezinye izilwane ezinesisu esisodwa azikwazi ukusebenzisa i-cellulose.Ukwengeza i-cellulase ekudleni kungathuthukisa ngempumelelo inani lokudla okunomsoco futhi kukhuthaze ukuzuza kwesisindo semfuyo nezinkukhu.
Imibandela yokusebenzisa:
Ibanga elisebenzayo: Izinga lokushisa: 40-55℃ PH: 4.5-6.5
Ibanga elingcono kakhulu: Izinga lokushisa: 45-50℃ PH: 4.8